General Cell Lines and Cell Cultures
Eurofins Discoverx ChemiScreen EP4 Prostanoid Receptor Membrane Preparation, 1 vial. (HTS142M)
The ChemiScreen EP4 Prostanoid Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of PTGER4 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay α1B Adrenergic Receptor Frozen Cells, 2 Vials of Cells. (HTS158RTA)
The Ready-to-Assay α1B Adrenergic Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1B GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRA1B alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of ADRA1B, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiBrite EP4 Prostanoid Receptor Stable Cell Line, 2 vials. (HTS142L)
The ChemiBrite EP4 Prostanoid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER4 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER4, the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay β3 Adrenergic Receptor Frozen Cells, 2 Vials of Cells. (HTS159RTA)
The Ready-to-Assay β3 Adrenergic Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRB3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRB3 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of ADRB3, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay BB3 Bombesin Receptor Frozen Cells, 2 Vials of Cells. (HTS160RTA)
The Ready-to-Assay BB3 Bombesin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. bombesin) based activation of BRS3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses BRS3 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of BRS3, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay GLP-1 Receptor Frozen Cells, 2 Vials of Cells. (HTS163LRTA)
The Ready-to-Assay GLP-1 Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. GLP-1) based activation of GLP1R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GLP1R alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of GLP1R, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay GLP-2 Receptor Frozen Cells, 2 Vials of Cells. (HTS164RTA)
The Ready-to-Assay GLP-2 Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. GLP-2) based activation of GLP2R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GLP2R alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of GLP2R, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ATCC Helicobacter pylori; 26695; genomic DNA
Genomic DNA isolated from This bacterial strain is also available as ATCC® 700392D-5™.
ATCC Staphylococcus aureus; Strain TCH959; genomic DNA
Genomic DNA isolated from Staphylococcus aureus subsp. aureus Strain TCH959. This bacterial strain is also available as ATCC Catalog No. BAA-1718™.
ATCC Streptococcus thermophilus; genomic DNA
Genomic DNA isolated from Streptococcus thermophilus. This bacterial strain is also available as ATCC Catalog No. BAA-250™.
ATCC Aspergillus nidulans; G00
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
ATCC Duck Embryo; Fibroblast; Pekin Duck (Anas platyrhynchus domesticus)
Duck embryo is a cell line exhibiting fibroblast morphology that was isolated from the embryo of a normal duck. This cell line was deposited by M Marcovici, J Prier, M Allen.
ATCC pRS424 phagemid; in E. coli
This is a YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. It contains promoters for in vitro RNA synthesis and priming sites useful for sequencing. It also contains the REP3 and FRT sequences necessary for high copy propagation in yeast. In S. cerevisiae, the number is about 20 per haploid cell. In the range of 1.5 +/? 1.7% of progeny per doubling. The order of the major features in this plasmid is: ori(fi) ? lacZ ? T7 promoter ? MCS (KpnI-SacI) ? T3 promoter ? lacI - ori (pMB1) ? ampR ? ori(2 micron) ? TRP1.
ATCC Thermus aquaticus
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